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. 2012 Nov 15;23(22):4383–4392. doi: 10.1091/mbc.E12-05-0365

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© 2012 Chen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Expression of CTTNBP2 and CTTNBP2NL in mouse tissues. (A) Characterization of the anti-CTTNBP2NL antibody by immunoblotting. Whole-cell extracts of COS cells transfected with HA-tagged CTTNBP2NL or the control vector were immunoblotted with anti-CTTNBP2NL antibodies. (B, C) Lysates from various mouse organs were immunoblotted using anti-CTTNBP2NL (B) and anti-CTTNBP2 (C) antibodies. B, brain; H, heart; K, kidney; L, lung; Lv, liver; M, muscle; Sk, skin; Sp, spleen; T, testis. (D) Real-time PCR analysis of the actual copy numbers of CTTNBP2 and CTTNBP2NL transcripts in total RNA extracted from different regions of the adult mouse brain. Cb, cerebellum; Cx, cerebral cortex; Hp, hippocampus; St, striatum.