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. 2012 Nov 15;23(22):4383–4392. doi: 10.1091/mbc.E12-05-0365

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© 2012 Chen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — CTTNBP2NL does not significantly regulate the density of dendritic spines in cultured hippocampal neurons. (A) The knockdown efficiency of NL-miR. Whole-cell extracts of COS cells cotransfected with HA-tagged CTTNBP2NL or Myc-tagged CTTNBP2 and Ctrl-miR or NL-miR, as indicated, were immunoblotted with the anti-HA antibody or the anti-Myc antibody. Tubulin was used as an internal control. (B, C) The effect of CTTNBP2NL on dendritic spines. Rat hippocampal neurons were transfected at 12 DIV and analyzed at 18 DIV. (B) Representative images of Ctrl-miR– or NL-miR–transfected neurons. Bottom, a higher magnification of the dendrites. Scale bars, 20 μm (top) and 5 μm (bottom). (C) Quantification of the density of dendritic spines in neurons transfected with Ctrl-miR, BP2-miR, BP2-miR and CTTNBP2NL, or NL-miR. More than 20 dendrites from 10 neurons were analyzed for each group.