FIGURE 8:

NMDA treatment induces the redistribution of striatin and zinedin. (A) At 18 DIV, cultured hippocampal neurons were stimulated with NMDA (100 μM) for 15 min, followed by staining with phalloidin, anti-CTTNBP2, and anti-striatin (top) or anti-zinedin (bottom) antibodies. (B) Using the same stimulation conditions as in A, we prepared whole-cell extracts of neurons for immunoblotting for striatin and zinedin, as indicated. Tubulin was used as an internal control. (C) An experiment similar to that performed as described in A was carried out, except that neurons were treated with 20 μM NMDA for 3 min. After 12 min of recovery, cells were harvested for immunostaining. (D) The total protein levels of striatin and zinedin in cultured hippocampal neurons were examined under the condition as described in C. Scale bar, 5 μm.