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. 2012 Nov 15;23(22):4402–4415. doi: 10.1091/mbc.E11-12-1015

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© 2012 Rockenbauch et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Deletion of TPRs in ChAPs only mildly affects protein expression levels. (A) Domain structure of the ChAP family members. Numbers indicate the first and last amino acid of the TPR domains. The same coloring scheme is used in all subsequent figures. (B) Expression of 9myc-tagged TPR mutants of Chs6p, under the native and the TEF promoter. Immunoblot of yeast lysates; Sec61p serves as loading control. Note that all mutants were generated chromosomally.