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. 2012 Nov 15;23(22):4402–4415. doi: 10.1091/mbc.E11-12-1015

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© 2012 Rockenbauch et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — The TPRs are interchangeable among ChAPs. (A) Chs6p bearing either TPR1–4 or TPR5 from Bch2p is fully functional. Chimeras in which TPRs from CHS6 were grafted into BCH2 (or vice versa) were created by delitto perfetto. Drop tests for calcofluor sensitivity were performed as in Figure 2. Transplanting TPR5 from Chs6p to Bch2p did not restore calcofluor sensitivity in a Δchs6 background. Blue, Chs6p domains; yellow, Bch2p domains. Δ refers to Δchs6. (B) Live fluorescence imaging of Chs3p-2GFP in the chimeras shown in A confirmed that the TPRs do not contribute to cargo specificity. Scale bar, 5 μm.