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. 2012 Nov 15;23(22):4402–4415. doi: 10.1091/mbc.E11-12-1015

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© 2012 Rockenbauch et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 9: — (A) The C-terminus of Chs3p is not sufficient for exomer-dependent cargo export. Replacement of the cytosolic domain of Kex2p, a TGN-resident protein, with the Chs3p C-terminus did not direct Kex2ΔC-Chs3CT-GFP to the plasma membrane. Kex2ΔC-Chs3CT-GFP was localized to the vacuolar lumen, whereas Kex2ΔC-GFP localized to the vacuolar rim, indicating an influence of the Chs3p C-terminus on Kex2p sorting. Kex2-GFP, C-terminally truncated Kex2ΔC-GFP, and the Kex2ΔC-Chs3CT-GFP chimera were chromosomally expressed. Sec7-dsRed was used as a TGN marker. Scale bar, 5 μm. (B) Kex2ΔC-Chs3CT-GFP does not traffic to the plasma membrane, and its localization is exomer independent. To assess potential trafficking through the plasma membrane, the localization of chromosomally expressed Kex2ΔC-GFP and the Kex2ΔC-Chs3CT-GFP was assessed in a Δend3, endocytosis-deficient strain. Kex2ΔC-GFP and Kex2ΔC-Chs3CT-GFP exomer-dependent localization was assessed in a Δchs5 strain. Scale bars, 5 μm.