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. 2012 Nov 13;7(11):e49426. doi: 10.1371/journal.pone.0049426

Figure 2. Enhancement of ENaC-dependent Na+ absorption and β- and γ-ENaC transcription by endiandrin A in HT-29/B6-GR cells.

Figure 2

HT-29/B6-GR cells were incubated with dexamethasone (1 µM) and/or TNF-α (500 IU/ml) and/or endiandrin A (20 µM) for 24 hours. (A) Measurement of ENaC-dependent Na+ absorption was determined as the drop in ISC after amiloride (100 µM) after 24 hours. Data are means ± s.e.m., n = 6–15, * P<0.05 compared with control, ### P<0.001 compared with dexamethasone alone, °° P<0.01 compared with dexamethasone + TNF-α 500 IU/ml. (B)/(C) Measurement of (B) β- or (C) γ-ENaC-mRNA. GAPDH was used for normalization of mRNA expression. (B) Real-time PCR of β-ENaC mRNA after 24 hours. Data are means ± s.e.m., n = 4–13, *** P<0.001 compared with dexamethasone alone, ## P<0.01 compared with dexamethasone + TNF-α 500 IU/ml. (C) Real-time PCR of γ-ENaC mRNA after 24 h. Data are means ± s.e.m., n = 4–12, * P<0.05 compared with dexamethasone alone, # P<0.05 compared with dexamethasone + TNF-α 500 IU/ml.