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. 2012 Nov 13;7(11):e49426. doi: 10.1371/journal.pone.0049426

Figure 6. Influence of Sgk-1 on the synergistic ENaC induction in HT-29/B6-GR cells.

Figure 6

(A) HT-29/B6-GR cells were transfected with psgk-1-short-Luc and incubated with dexamethasone (1 µM) and/or TNF-α (500 IU/ml) and/or endiandrin A (20 µM) for 24 hours. Data are given as normalized relative luciferase activity and as means ± s.e.m., n = 6–14 * P<0.05 compared to dexamethasone alone, # P<0.05 compared with dexamethasone + TNF-α 500 IU/ml. (B) Measurement of sgk-1 mRNA after 24 h. GAPDH was used for normalization of mRNA expression. Data are means ± s.e.m., n = 5–8, ** P<0.01 compared with dexamethasone treatment, ## P<0.01 compared with dexamethasone + TNF-α 500 IU/ml, °°° P<0.001 compared with TNF-α 500 IU/ml alone. (C) Measurement of sgk-1 mRNA pre-incubation with the indicated inhibitors (10 µM) for 1 hour before addition of dexamethasone (1 µM) and/or TNF-α (500 IU/ml) and/or endiandrin A (20 µM) for 24 hours. Data are means ± s.e.m., n = 5–7, *** P<0.001 compared with dexamethasone + TNF-α 500 IU/ml, # P<0.05 compared to dexamethasone + TNF-α 500 IU/ml + U0126 inhibitor.