Figure 7. Endiandrin A amplified dexamethasone-induced ENaC-dependent Na⁺ absorption under inflammatory conditions in rat distal colon.

(A) Time course of electrogenic Na⁺ transport in rat distal colon. Experiments were performed as described under methods. (B) Electrogenic Na⁺ transport in rat distal colon. Tissue was mounted into Ussing chambers and stimulated with dexamethasone (1 µM) and/or TNF-α (10,000 IU/ml) and/or endiandrin A (20 µM) for 14 hours after a pre-incubation with IFN-γ (1,000 IU/ml) for 1 hour. ENaC-dependent Na⁺ absorption was determined as the drop in I_SC within 10 minutes after addition of amiloride (100 µM). Results are given as means ± s.e.m., n = 4–18, **P<0.01 compared with dexamethasone treatment, ^# P<0.05 compared with dexamethasone + TNF-α 10,000 IU/ml.