Table 2. Summary of in vivo metastasis data, targeting the adhesion molecules described in Table �1.

Adhesion molecule	In vivo model	Metastasis phenotype
FAK	Intravenous injection of FAK−/− tumor cells82	Failed to form lung tumors; cells retained in the lung capillary bed were rounded and lacked membrane extensions into the vessel
	Activated ErbB2 mammary tumor model with epithelial-specific FAK deletion69	Metastatic lung tumors all negative for cells with homozygous deletion of FAK
	Mammary epithelium-specific deletion of FAK in MMTV-PyVmT Mouse Tumor Model70	Metastatic lung tumors all negative for cells with homozygous deletion of FAK
	Mammary epithelium-specific deletion of FAK in MMTV-PyVmT Mouse Tumor Model71	Reduced lung tumor metastases
	Orthotopic injection of pancreatic cancer cells treated with FAK siRNA73	Prevented formation of liver metastases
p130Cas	Mammary tumor model—injection of cells expressing inducible p130Cas shRNA77	Inhibits lung colonization
	Athymic nude mice injected sub-cutaneously with p130Cas−/− fibroblasts transformed with oncogenic Src and expressing p130Cas78	Exogenous p130Cas expression increased formation of metastatic lung tumors after surgical removal of primary tumors; authors comment that “the capacity of the cells to invade through matrigel was strongly correlated with their capacity to invade and metastasize in vivo”
Vinculin	Exogenous vinculin expression in highly metastatic rat adenocarcinoma injected into foot pad80	Highest levels of vinculin expression suppressed formation of lung metastases, low to moderate expressors formed tumors in lymph nodes close to injection site but failed to form lung metastases
NEDD9	Mammary epithelium-specific deletion of NEDD9 in MMTV-PyVmT Mouse Tumor Model67	Trend to fewer lung metastases
	Tail vein injection of NEDD9-null primary tumors8	Tumors formed of null-cell lines exhibited increased aggressiveness, with all injected mice generating secondary tumors
Talin	Tail vein injections of prostate cancer cell lines treated with talin shRNA84	Reduced numbers of metastatic lung lesions
αvβ3	Orthotopic injection into mammary fat pad with mammary carcinoma cell line expressing exogenous β376	Drove unique formation of bone metastases; authors show increased haptotactic and chemotactic response to bone-matrix proteins and soluble factors
	MDA-MB-435 breast cancer cells expressing constitutively active αvβ3 injected into mouse tail vein81	Enhanced lung colonization
	Intravenous injection of metastatic αvβ3 negative melanoma cells expressing exogenous β391	Re-expression of β3 in metastatic, β3 negative lines reduced lung colonization
β1 integrin	Conditional deletion of β1 integrin from mammary epithelia, crossed with MMTV/activated erbB272	Significantly reduced formation of lung metastases
	Orthotopic injection of pancreatic cancer cells treated with β1 integrin siRNA74	Absence of any metastatic tumors; controls treated with α2 or α3 integrin subunit siRNA displayed metastatic tumors
	Conditional deletion of β1 integrin from pancreatic β cells crossed with Rip1Tag2 mice68	Loss of β1 expression induced increased tumor cell emboli in the lymphatic vasculature but no metastasis formation; similarly, tail vein injections of β tumor cells lacking β1 integrin expression did not form metastases
	Ras-myc transformed β1 null ES cells injected sub-cutaneously79	Reduced numbers and size of metastatic foci in the lung
α5β1 integrin	HT-29 colon cancer cells expressing exogenous α5 integrin injected intravenously85	Significantly reduced lung and extrapulmonary metastases
	Lewis Lung Carcinoma cells expressing α5 shRNA injected into tail vein83	Fewer lung tumors