Figure 3. Knockdown HDAC6 with shRNA increases EGFR degradation.

(A) and (B) Western blotting analysis of the expression of HDAC6, EGFR and acetyl-α-tubulin in Pkd1 wild-type (WT) MEK cells (A) and Pkd1^null/null (Null) MEK cells (B) transduced with lentiviral vector pGIPZ-shHDAC6 mediated HDAC6 shRNA or control lentiviral empty vector. The levels of EGFR were reduced and the levels of acetyl-α-tubulin were increased in HDAC6 knockdown cells. (C) EGFR expression at mRNA level in Pkd1 mutant (Null) MEK cells with HDAC6 shRNA or vector (control) was analyzed by real-time PCR. Knockdown HDAC6 with shRNA did not affect the transcription of EGFR. Average values from triplicated experiments were shown.