Figure 8. Microtubule stability affected EGF induced EGFR degradation.

(A) and (B) Influence of nocodazole on EGF-induced EGFR degradation. Pkd1 wild-type (WT) MEK cells (A) and Pkd1^null/null (Null) MEK cells (B) were pretreated with 10 µg/ml cycloheximide for 2 hours. Pretreated cells were treated with 0.2 µg/ml nocodazole and/or 100 ng/ml EGF for another 2 hours. EGF-induced EGFR degradation was strikingly attenuated after co-treatment with EGF and nocodazole, which disturbed the stability of microtubule. * p < 0.05. ** p < 0.01.