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. 2012 Nov 13;7(11):e49418. doi: 10.1371/journal.pone.0049418

Figure 9. Treatment with nocodazole decreased EGFR intracellular transport.

Figure 9

(A) Pkd1 wild type MEK cells were serum-starved and preteated with nocodazole (0.2 µg/ml) for 30 min. The pretreated cells were further treated with nocodazole and 80 ng/ml EGF-Alexa Fluor 488 for indicated periods of time. Then, cells were stained with anti-EEA1 antibody. Localization of EGF-Alexa Fluor 488 to the EEA1-containing vesicles was analyzed. * p < 0.05. (B) Pkd1 mutant (Null) MEK cells were treated, processed for immunostaining, and analyzed as in (A). * p < 0.05. (C) Pkd1 wild type MEK cells were treated as in (A) and then processed for immunostaining with anti-LAMP2 antibodies. Co-localization of EGF-Alexa Fluor 488 to LAMP-2-containing vesicles was examined. * p < 0.05. (D) Pkd1 mutant (Null) MEK cells were treated, processed for immunostaining with anti-LAMP2 antibodies, and analyzed as in (C). * p < 0.05.