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. 2012 Nov 13;7(11):e49323. doi: 10.1371/journal.pone.0049323

Figure 1. Schematic representation of the piggyBac-based BmNPV ie1 promoter reporter constructs.

Figure 1

This construct was designated pBac[BmNPV ie1-EGFP, 3xP3-DsRed]. A fragment containing the sequences –631 to –2 bp upstream of the codon encoding the translational start site of BmNPV ie1 was used as the BmNPV ie1 promoter and to drive expression of EGFP. DsRed was under the control of 3xP3 and was used as the transformation marker. Abbreviations: ITR, inverted terminal repeats of piggyBac; hsp70 polyA, hsp70 polyadenylation signal; SV40 polyA, SV40 polyadenylation signal.