Table 1. Primers used in this study.
PCR | Primer | Sequence | Productsize (bp) | PCRcycles | Annealingtemp. (°C) |
RT-PCR | CBS-F | CAGAGGATAAGGAAGCCAAG | 192 | 35 | 55 |
CBS-R | TCCCAATCTTGTTGATTCTGAC | ||||
MSP | CBSm3 | CGAGATATTGGTCGGCGTC | 129 | 40 | 60 |
CBSm4 | CCCCGACTACGACGAAACG | ||||
CBSu3 | TTATGAGATATTGGTTGGTGTT | 135 | 41 | 58 | |
CBSu4 | TACCCCAACTACAACAAAACA |