Figure 3. SMEPT offers several arms of control over the rate of generation and the overall amount of the product generated by the enzyme containing substrates: concentration of the enzyme in the gel (a), concentration of the added substrate (b) and time of enzymatic conversion (c).

Experimental conditions: (a) FdG, 2,5 µg/mL, 30 min reaction time; (b): 1 g/L β-Glu in polymer solution, 30 min reaction time; c) FdG: 2,5 µg/mL; β-Glu: 1 (top), 0.1 (middle) and 0.01 (bottom) g/L, respectively. Presented results (mean ± st.dev.) are average over at least 3 independent experiments, 3 replicates each.