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. 2012 Nov 13;7(11):e48587. doi: 10.1371/journal.pone.0048587

Figure 4. The effect of the UPR pathways on the expression of the Ufm1 system.

Figure 4

A. Immunoblotting of MEF cell lysates using Ufm1, Uba5, RCAD/Ufl1 and C53/LZAP antibodies. GAPDH was used as a loading control. Relative ratios of proteins were measured against GAPDH using Image J software. B. The mRNA levels of Ufm1, Uba5, RCAD/Ufl1 and C53/LZAP in wild-type and Xbp-1−/− MEF cells that were treated with ER stress-inducing agents (TG, 0.5 µM for 16 hour; TM, 10 µM for 16 hours; and BFA, 0.5 µg/ml for 16 hours). C. The mRNA levels of Ufm1, Uba5, RCAD/Ufl1 and C53/LZAP in wild-type and PERK−/− MEF cells that were treated with ER stress-inducing agents. The results represented mean ± SD. *p value <0.01.