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. 2012 Nov 13;7(11):e49105. doi: 10.1371/journal.pone.0049105

Figure 4. Imaginal Disc Damage Delays the Mid-Third Instar Transition.

Figure 4

(A–F) Timing of the mid-third instar transition (as measured by Sgs3GFP expression) in larvae that were heat-treated for 24 hours at 172 hours AEL to induce tissue damage in wing discs. (A–C) Sgs3GFP expression in Non-Ablating (w*; TM6, Tb1, tubGAL80/Sgs3GFP) larvae. (A) Sgs3GFP expression is absent in most larvae at 164 hours AEL, just before heat treatment (Sgs3GFP+ = 3.6±4.7%; n = 28). Sgs3GFP is expressed at high levels after heat treatment in salivary glands at (B) 197 hours AEL (Sgs3GFP+ = 77.3±0.6%; n = 22), at (C) 215 hours AEL (Sgs3GFP+ = 91.7±7.9%; n = 12) and at 236 hours AEL (Sgs3GFP+ = 100±0.0%; n = 19) – data not shown. (D–F) Sgs3GFP expression in Ablating (w*; rnGAL4, UAS-egr, tubGAL80ts/Sgs3GFP) larvae. (D) Sgs3GFP expression is absent in most larvae at 164 hours AEL, just before heat treatment (Sgs3GFP+ = 2.6±3.1%; n = 38). (E) Sgs3GFP expression is detected at low levels at 197 hours AEL in only a small fraction of larvae examined (Sgs3GFP+ = 10.0±13.3%; n = 30). (F) High levels of Sgs3GFP expression is visible at 215 hours AEL (Sgs3GFP+ = 53.9±37.9%; n = 26) and at 236 hours AEL (Sgs3GFP+ = 75.0±1.3%; n = 28) – data not shown.