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. 2012 Nov 13;7(11):e49105. doi: 10.1371/journal.pone.0049105

Figure 5. Damage to Wing Imaginal Discs Delays Pupariation and Adult Eclosion.

Figure 5

(A–F) Timing of pupariation and adult eclosion following induction of cell ablation in the wing disc at the indicated time. Fraction of larvae that had (A, C, E) pupariated or (B, D, F) eclosed as adults are plotted relative to time in hours AEL for Ablating (Red - w*; rnGAL4, UAS-egr, tubGAL80ts/Sgs3GFP) and Non-Ablating (Blue - w*; TM6, Tb1, tubGAL80/Sgs3GFP) larvae. n = 3 independent populations (30 larvae each) were assayed for each ablation time. (A–B) Timing of (A) pupariation and (B) adult eclosion for larvae heat-treated at 173 hours AEL. Mean pupariation times are 286 and 227 hours AEL for Ablating and Non-ablating larvae, respectively. Mean eclosion times are 504 and 440 hours AEL for Ablating and Non-ablating larvae, respectively. Similar results were obtained with larvae heat-treated at 150, 162, or 184 hours AEL (data not shown). (C–D) Timing of (C) pupariation and (D) adult eclosion for larvae heat-treated at 198 hours AEL. Mean pupariation times are 279 and 230 hours AEL for ablating and non-ablating larvae, respectively. Mean eclosion times are 488 and 409 hours AEL for ablating and non-ablating larvae, respectively. Similar results were obtained with larvae heat-treated at 190 hours AEL (data not shown). (E-F) Timing of (E) pupariation and (F) adult eclosion for larvae heat-treated at 223 hours AEL. Mean pupariation times are 263 and 265 hours AEL for ablating and non-ablating larvae, respectively. Mean eclosion times are 419 and 411 hours AEL for ablating and non-ablating larvae, respectively. Similar results were obtained with larvae heat-treated at 213 and 220 hours AEL (data not shown).