Table 2. ARGINASE 1 Panel.
Antigen | Fluorophore | Clone | Isotype | Produced by | Product code | Volume used (µL) | Quantity (µg) |
CD15 | PE | H198 | mouse IgM | BD Pharmingen | 555402 | 20 | NP |
CD13 | APC-Cy7 | WM15 | mouse IgG1 | Biolegend | 301710 | 7 | NP |
CD16 | eFluor 450 | eBioCB16 | mouse IgG1 | eBioscience | 48–0168 | 3 | 0.15 |
CD63 | FITC | CLBGran/12 | mouse IgG1 | Beckman Coulter | IM1165U | 14 | NP |
Arginase 1 | Alexa Fluor® 647 | 6G3 | mouse IgG1 | Hycult biotech. | HM2162 | 2.75 | 0.275 |
LDGs and NDGs were isolated as described in materials and methods and the expression levels of phenotypic markers were determined by flow cytometry.
NP = Not provided by manufacturer.