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Confocal fluorescence images of HCT 116 cells incubated with 2PA probe 1 encapsulated in Pluronic® F-127 (20 μM, 1 h) and Lysotracker Red (75 nM, 1 h). DIC (a), one-photon fluorescence image showing Lysotracker Red (b) and 2PA probe 1 encapsulated in Pluronic® F-127 micelles (c). (d) Colocalization (overlay of B and C). 10 μm scale bar.
