Table II.

Half-life of the D1 polypeptide in the site-specific D-E loop mutants of Synechocystis sp. PCC 6803 at high-light and growth-light conditions

Strain	Half-Life
	High light	Growth lighta
	min
AR	26  ± 3	198  ± 18
PD	45  ± 3	120  ± 6
CD	56  ± 2	108  ± 18
PCD	53  ± 5	162  ± 18

Cells were pulse labeled with l-[³⁵S]Met and the radioactivity was chased at either high-light (1500 μmol photons m⁻² s⁻¹) or growth-light (40 μmol photons m⁻² s⁻¹) conditions. Fluorograms were analyzed with a laser densitometer, and the results were fitted to first-order kinetics to determine the half-life (t_½) of the D1 polypeptide. Results are the means ± se of two to four independent experiments.

^aData were taken from Mulo et al. (1997).