Figure 1.

Fibrin accumulation in both AnxA2^−/− and hyperhomocysteinemic mice. Kidney sections from AnxA2^+/+ mice on normal chow (a and b), from AnxA2^−/− mice on normal chow (c), or from AnxA2^+/+ mice on a high methionine diet (d). Sections from heparin-treated, highly perfused animals were stained with a polyclonal rabbit antifibrin(ogen)-specific antibody using either immunoperoxidase plus diaminobenzidine (brown immunoreaction product) with hematoxylin counterstaining [5] (a and c), or biotinylated goat anti-rabbit IgG followed by Alexa 568-conjugated streptavidin (b and d); red staining represents Alexa 568, blue represents DAPI nuclear staining, and green is tissue autofluorescence [6].