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. Author manuscript; available in PMC: 2013 Oct 30.
Published in final edited form as: Mol Biosyst. 2012 Oct 30;8(12):3305–3313. doi: 10.1039/c2mb25341k

Fig. 4.

Fig. 4

Formation of 1,2′-di-acetyl-NEA by Eis_Ava monitored by a TLC assay and confirmed by NMR spectroscopy. Lanes 1–6: a time course showing the mono- and di-acetyl-NEA products of the Eis_Ava reaction. Lanes 7–12: controls for mono- and di-acetylation of NEA by AAC(2′)-Ic, AAC(3)-IV, and AAC(6′)-Ie used individually or sequentially.