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. 2012 Sep 14;120(19):4038–4048. doi: 10.1182/blood-2012-05-429050

Figure 2.

Figure 2

AML1-ETO is recruited to ETS factor binding sites. (A-B). Re-ChIP experiments validating AML1-ETO and ERG binding to the same locus. Five binding sites were selected and validated for AML1-ETO/ERG binding by re-ChIP using either ERG antibodies in the first round of ChIP followed by a second round using AML1-ETO and no antibodies (A) or AML1-ETO antibodies in the first round of ChIP followed by a second round using ERG and no antibodies (B). (C) Coimmunoprecipitation of AML1-ETO with ERG. Immunoprecipitations were performed in SKNO-1 cells using IgG and ERG antibodies and analyzed by Western using RNAPII and AML1-ETO antibodies. (D) ChIP-seq using U937 cells expressing (+ zinc) or not expressing (− zinc) AML1-ETO. Overview of the SKI AML1-ETO binding site in U937 AML1-ETO cells. Blue represents the AE ChIP-seq data; and pink, the FLI1 data. (E) Intensity plot showing the tag density of AML1-ETO and FLI1 tags within a 10-kb window around AML1-ETO binding sites in U937 AML1-ETO cells treated or untreated with zinc.