Table 1.
Microarray analyses of genes involved in the anthranilate degradation pathway
| Gene1 | Name | Operon1 | EC | Fold change | Description if available | |
|---|---|---|---|---|---|---|
| Anthranilate degradation | PA0226 | pcaI | 47 | 2.8.3.6 | 3.7 ± 0.7 | 3-Oxoadipate CoA-transferase, subunit A |
| PA0227 | pcaJ | 47 | 2.8.3.6 | 1.6 ± 0.3 | 3-Oxoadipate CoA-transferase, subunit B | |
| PA0228 | pcaF | 47 | 2.3.1.174 | ND | β-Ketoadipyl CoA thiolase PcaF | |
| PA0229 | pcaT | 48 | 1.2 ± 0.2 | Dicarboxylic acid transporter PcaT | ||
| PA0230 | pcaB | 48 | 5.5.1.2 | 2.7 ± 0.8 | 3-Carboxy-cis,cis-muconate cycloisomerase | |
| PA0231 | pcaD | 48 | 3.1.1.24 | 3.3 ± 1.4 | β-Ketoadipate enol-lactone hydrolase | |
| PA0232 | pcaC | 48 | 4.1.1.44 | 4.8 ± 2.2 | γ-Carboxymuconolactone decarboxylase | |
| PA2507 | catA | 527 | 1.13.11.1 | 3.3 ± 1.0 | Catechol 1,2-dioxygenase | |
| PA2508 | catC | 527 | 5.3.3.4 | 2.4 ± 0.4 | Muconolactone delta-isomerase | |
| PA2509 | catB | 527 | 5.5.1.1 | 2.9 ± 1.5 | Muconate cycloisomerase I | |
| PA2512 | antA | 528 | 1.14.12.1 | 5.5 ± 2.6 | Anthranilate dioxygenase large subunit | |
| PA2513 | antB | 528 | 1.14.12.1 | 20.5 ± 9.8 | Anthranilate dioxygenase small subunit | |
| PA2514 | antC | 528 | 1.14.12.1 | 20.8 ± 9.5 | Anthranilate dioxygenase reductase | |
| Anthranilate synthesis via the Kynurenine pathway | PA2080 | kynU | 438 | 3.7.1.3 | 1.1 ± 0.1 | Kynureninase |
| PA2081 | kynB | 438 | 3.5.1.9 | 0.8 ± 0.2 | Kynurenine formamidase | |
| PA2579 | kynA | 1.13.11.11 | ND | L-Tryptophan:oxygen 2,3-oxidoreductase | ||
| Anthranilate synthesis via chorismate | PA0609 | trpE | 135 | 4.1.3.27 | 0.8 ± 0.1 | Anthranilate synthase component I |
| PA0649 | trpG | 146 | 4.1.3.27 | 0.9 ± 0.1 | Anthranilate synthase component II | |
| PA1001 | phnA | 215 | 4.1.3.27 | 0.9 ± 0.2 | Anthranilate synthase component I | |
| PA1002 | phnB | 215 | 4.1.3.27 | 1.0 ± 0.2 | Anthranilate synthase component II | |
| Pseudomonas quinolone signal (PQS) | PA1003 | pqsR | ND | LysR, transcriptional regulator | ||
| PA0996 | pqsA | 214 | 0.7 ± 0.1 | |||
| PA0997 | pqsB | 214 | 0.7 ± 0.1 | |||
| PA0998 | pqsC | 214 | 0.4 ± 0.1 | 3-Oxoacyl-(acyl-carrier-protein) synthase | ||
| PA0999 | pqsD | 214 | 0.5 ± 0.1 | 3-Oxoacyl-(acyl carrier protein) synthase III | ||
| PA1000 | pqsE | 214 | 0.6 ± 0.1 |
Fold change corresponds to the SC24/PC24 ratios. Results are the mean value of the nine ratios of the three biological SC24 replicates and the three biological PC24 replicates ± SD.
1
IDs of genes and operons are from the Pseudomonas Genome Database (http://www.pseudomonas.com).
ND, not determined because the intensity of the corresponding probe did not fulfill our criteria for quantification.
Bold value indicates the greater than 2.