Figure 7.

Electron micrographs of Golgi stacks from auxin-deprived BY-2 cells. Cells were fixed at 14 h (a), 1.5 d (b), and 4 d (c) after auxin deprivation. The cis, medial, and trans cisternae are labeled in b and c. Intercisternal filaments are indicated by arrowheads. After 14 h one could already see hypertrophied cisternal margins and a clustering of the stacks. After 1.5 d an increase in the size of the cisternal margins and an increase in the staining of intercisternal elements could be seen. After 3 to 4 d densely staining material appeared in the medial cisternae, and misshapen or thickened regions appeared in the interior of the Golgi stack. Bars = 0.2 μm.