Figure 9.

SDS-PAGE (a) and western blots (b–d) of secreted material from control (+) and auxin-deprived (−) BY-2 cells. Molecular mass in kilodaltons is indicated to the left of the gels. All lanes were loaded with equal amounts of proteins. Western blots were probed with the lectins concanavalin A (Con A) and UEA-1 and with the monoclonal antibody JIM 13. b, Concanavalin A, a Man-specific lectin, showed minor changes in a set of N-linked glycoproteins between 45 and 97.5 kD. c, UEA-1, a Fuc-specific lectin, showed a major increase in a single, diffuse band between 100 and 200 kD. d, JIM 13, a marker of root-cap differentiation, showed an increase in staining between 40 and 180 kD. All gel images were digitized.