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. 2012 Oct 16;4(10):2137–2161. doi: 10.3390/v4102137

Figure 5.

Figure 5

D471G amino acid substitution does not affect dsRNA binding of LCMV-NP. Human 293T cells were co-transfected with 2.5 μg of the indicated pC-LCMV-NP wt or amino acid mutants HA-tagged. pC-LCMV-Z HA-tagged was used as negative control for binding. At 48 hpt, cell lysates were prepared and analyzed for NP expression levels by WB using an anti-HA antibody (A). GAPDH was used as a loading control. Cell lysates were used in pull-down assays with poly I:C bound to sepharose beads and immunoprecipitates analyzed by WB (B). Numbers at the bottom of each WB lane represent the quantification of band intensities normalized to wt NP lane, as described in material and methods.