Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 16;4(10):2137–2161. doi: 10.3390/v4102137

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Dominant negative effect of LCMV-NP D471G on viral replication and transcription. BHK-21 cells were co-transfected in triplicate with 0.5 μg of pPolI GFP-Pur/Gluc, 0.6 μg of pC-L, and the indicated amounts (ng) of pC-LCMV-NP HA-tagged (NP); together with empty pC (Empty) or pC LCMV-NP D471G HA-tagged (D471G), and 0.1μg of the pSV40-Cluc expression vector to normalize transfection efficiencies. At 48 hpt, MG activity was determined by GFP expression (A) and luciferase activity from TCS (B). Reporter gene activation is shown as induction over an empty pC vector-transfected control.