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SDS–PAGE and immunoblotting analysis of the RC47-His and PSII-His complexes isolated from Synechocystis sp. PCC 6803. (a) Coomassie-stained 18% (w/v) PAA SDS–PAGE gel and (b) immunoblotting analyses with indicated antibodies. Thylakoid membranes (0.5 µg Chl a), the RC47-His protein complex (1 µg Chl a) and non-oxygen-evolving (inactive) as well as oxygen-evolving (active) PSII core complexes (1 µg Chl a each) were loaded on the gels.
