(a–g) Protein analysis of the TLA1 RNAi transformants and the CC503 wild-type strain. (a) SDS–PAGE of total cell protein extracts from the TLA1 RNAi transformants (Ri6, Ri8 and Ri9) and the WT strain. Lanes were loaded on an equal protein basis (25 µg of protein per lane). (b–g) Western blot analysis of C. reinhardtii total cell protein extracts. Lanes, loaded as in (a) were probed with (b) TLA1-specific, (c) LHC-specific, (d) D1 reaction centre protein-specific, (e) D2 reaction centre protein-specific, (f) VIPP1-specific and (g) NAB1-specific polyclonal antibodies.