(a,b) Cell fractionation and Western blot analysis of Chlamydomonas reinhardtii soluble and membrane fractions, probed with TLA1-specific polyclonal antibodies. (a) SDS–PAGE of total cell protein extracts from different cell fractions of CC503 (WT) strain: lane T, total cell extract; lane S, soluble fraction and lane M, membrane fraction. ST denotes standard molecular weight protein markers. (b) Western blot analysis of the total, soluble and membrane fractions of C. reinhardtii. Lanes were loaded as in (a) and probed with the TLA1-specific antibodies.