Figure 5.

Immunohistochemical localization of nENaCα and Na⁺, K⁺-ATPase in the gills, kidney and rectum, using a polyclonal Bufo ENaCα antibody or a polyclonal Na⁺, K⁺-ATPase antibody. (a) Immunoreactivity (green fluorescence) for nENaCα was observed in the apical cell membrane (arrows) in the epithelial cells of gill lamella. (b) Apical cell membranes (arrows) of some cells in the late distal tubules of the nephron were also immunoreactive. (c) Immunoreactivity (green fluorescence) was observed in the apical membrane of rectum epithelium. (d) Na⁺, K⁺-ATPase immunoreactivity was observed in the basolateral cell membrane of the cells along the early distal (ED) to the late distal tubules (LD) in the kidney. Scale bars, 20 µm.