Fig 2.
Luciferase activity of cultures treated with PPMOs. Growing cultures were treated for 7 h without PPMO (no PPMO) or with 3 concentrations of a PPMO [(RFF)3RXB-Luc] targeted to a luciferase reporter gene or a scrambled-base-sequence control [(RFF)3RXB-Scr]. After 7 h, samples of each culture were measured for luciferase activity by luminometry. The experiment was repeated 3 times, and the error bars indicate standard deviations. *, a highly significant (P < 0.01) difference compared to cultures of PR200.1 with the same concentrations of (RFF)3RXB-Luc, the cultures of W3110 with the same concentrations of (RFF)3RXB-Scr, or the culture without PPMO.