Table 1.
MIC of AcpP PPMOs in pure cultures of E. coli
| Motif, PPMO no.a | Conjugated peptideb | MIC (μM [μg/ml]) |
|
|---|---|---|---|
| W3110 | PR200.1 | ||
| Motif 1 (C-N-N) | |||
| NG-05-0200 | RFFRFFRFFRXB | 2.5 (14) | 40 (222) |
| NG-05-0653 | DRDFDFDRDFDFDRDFDFDRXB | 2.5 (14) | 40 (222) |
| NG-23-248 | RXXRXXRXXRXB | 20 (102) | 80 (204) |
| NG-06-0199 | KFFKFFKFFKXB | 10 (54) | 80 (435) |
| Motif 2 (C-N), NG-06-0073 | RXRXRXRXRXRXB | 1.25 (7) | 1.25 (7) |
| Motif 3 (C-N-C) | |||
| NG-06-0076 | RXRRXRRXRRXRXB | 1.25 (7) | 1.25 (7) |
| NG-07-0795 | RFRRFRRFRRFRXB | 1 (6) | 16 (94) |
| No motif, NG-05-0246 | RTRTRFLRRTXB | 20 (111) | 40 (111) |
a
Motif 1 is cationic-nonpolar-nonpolar (C-N-N). Motif 2 is cationic-nonpolar (C-N). Motif 3 is cationic-nonpolar-cationic (C-N-C).
b
X is 6-aminohexanoic acid, B is β-alanine, O is ornithine, and D indicates the isomeric form or the residue that follows.