Table 3.
MICs of standard antibiotics and PPMOs using transposome mutant RR3, isogenic parent strain XL1-Blue MRF′, and their sbmA complemented strains
| PPMO no. | Antibiotic or PPMO | MIC (μM [μg/ml]) |
|||
|---|---|---|---|---|---|
| XL1-Blue MRF′ | RR3 | XL1-Blue MRF′ (pSbmA) | RR3(pSbmA) | ||
| Polymyxin B | 0.8 (1) | 0.8 (1) | 0.8 (1) | 0.8 (1) | |
| Colistin | 0.9 (1) | 0.9 (1) | 0.9 (1) | 0.9 (1) | |
| Erythromycin | 34 (25) | 34 (25) | 34 (25) | 34 (25) | |
| Rifampin | 6 (5) | 6 (5) | 6 (5) | 6 (5) | |
| Bleomycin | 0.2 (0.25) | 0.7 (1) | 0.01 (0.016) | 0.01 (0.016) | |
| Phleomycin | 0.3 (0.5) | 1.3 (2) | 0.02 (0.03) | 0.02 (0.03) | |
| NG-05-0200 | (RFF)3RXB-AcpP | 2 (11) | 64 (355) | 2 (11) | 2 (11) |
| NG-05-0653 | [d-(RFF)3R]XB-AcpP | 2 (11) | 64 (355) | 2 (11) | 2 (11) |
| NG-06-0076 | (RXR)4XB-AcpP | 1 (6) | 8 (48) | 0.1 (0.6) | 0.1 (0.6) |
| NG-05-0655 | (RFF)3RXB-Scr | >128 (>714) | >128 (>714) | >128 (>714) | >128 (>714) |
| NG-06-0078 | (RXR)4XB-Scr | >128 (>714) | >128 (>714) | >128 (>714) | >128 (>714) |