Fig 1.

Schematic representation of the ergosterol biosynthetic pathway in C. glabrata. (A) Sterol intermediates (boxed with a single line) that accumulate due to perturbations in C8-isomerase (ERG2 protein) activity. (B) Sterol intermediates that accumulate following azole inhibition of sterol 14α-demethylase (ERG11 protein). The fungistatic sterol 14α-methylergosta-8,24(28)-dien-3β,6α-diol is highlighted (boxed with a double line). Broken arrows, multiple enzymatic steps; unbroken arrows, single enzymatic step. ERG3, ERG4, ERG5, ERG6, ERG25, ERG26, and ERG27 encode C5-desaturase, C24-reductase, C22-desaturase, C24-methyl transferase, C4-methyloxidase, C4-decarboxylase, and C3-ketoreductase, respectively.