Table 3.

Concentration-dependent bactericidal antisense effects of anti-rpoA and anti-rpoD^a

Antisense PNA or water	Effect at PNA concn of:
	5 μM		10 μM		15 μM
	Log CFU	Log CFU reduction	Log CFU	Log CFU reduction	Log CFU	Log CFU reduction
Anti-rpoA	5.74 ± 0.68	3.16*	5.24 ± 0.20	3.63*	0	8.92*
Anti-rpoD	6.74 ± 0.33	2.16*	4.21 ± 0.03	4.66*	0	8.92*
Control	8.90 ± 0.03	0	8.53 ± 0.11	0.34	9.01 ± 0.05	−0.09
Water	8.90 ± 0.01	0	8.87 ± 0.05	0	8.92 ± 0.05	0

^aPNAs were incubated with ∼2 × 10⁴ CFU/ml S. enterica serovar Typhimurium in TSB for 8 h. Samples were serially diluted, and CFU/ml were estimated by plating onto TSA. The CFU represent the means ± standard errors from at least 2 independent experiments. Statistical analysis was performed for comparisons between groups with Student's t tests. The statistical significance level for the experiments was defined as P < 0.05 (asterisks).