Table 4.

Concentration-dependent bactericidal antisense effects of PNAs in cell culture^a

Antisense PNA	Effect at PNA concn of:
	5 μM		30 μM
	Log CFU	Log CFU reduction	Log CFU	Log CFU reduction
Anti-ligA	3.53 ± 0.05	−0.56	3.9 ± 0.04	0.51*
Anti-rpoA	3.51 ± 0.14	−0.54	3.89 ± 0.09	0.52*
Anti-rpoD	3.02 ± 0.20	−0.05	4.00 ± 0.02	0.41*
Anti-engA	2.85 ± 0.09	0.12	3.84 ± 0.06	0.57*
Anti-tsf	2.76 ± 0.11	−0.21	3.5 ± 0.02	0.91*
Anti-kdtA	2.80 ± 0.19	−0.17	3.68 ± 0.03	0.73*
Control	2.97 ± 0.22	00	4.41 ± 0.03	0

^aPNAs were incubated with S. enterica serovar Typhimurium-infected J774 cells for 4 h. The number of viable bacterial cells was determined by lysing the J774A.1 cells and counting the number of subsequent colonies on TSA plates. Values represent the means ± standard errors of CFU from at least 2 independent experiments. Statistical analysis was performed for comparisons between groups with Student's t tests. The statistical significance level for the experiments was defined as P < 0.05 (asterisks).