Table 3.
Monosaccharide composition of the parental, mutant, and revertant strain conidial cell wallsa
| Strain | Alkali-insoluble (AI) fraction |
Alkali-soluble (AS) fraction |
AI/AS | |||||
|---|---|---|---|---|---|---|---|---|
| Man | Glu | Gal | GlcN | Man | Glu | Gal | ||
| WT | 8.6 ± 0.6 | 53.6 ± 0.9 | 2.9 ± 0.4 | 4.1 ± 0.2 | 4.5 ± 0.5 | 24.4 ± 1.1 | 1.9 ± 0.2 | 2.25 ± 0.1 |
| ΔcsmA | 3.8 ± 0.2 | 42.6 ± 1.7 | 1.4 ± 0.4 | 1.3 ± 0.1 | 6.0 ± 0.8 | 43.0 ± 1.0 | 2.0 ± 0.7 | 0.96 ± 0.1 |
| ΔcsmB | 4.6 ± 1.0 | 43.6 ± 0.9 | 2.5 ± 0.8 | 3.5 ± 0.2 | 4.7 ± 0.9 | 39.6 ± 0.9 | 1.5 ± 0.5 | 1.18 ± 0.1 |
| ΔcsmA ΔcsmB | 4.3 ± 0.4 | 39.4 ± 0.9 | 2.9 ± 0.4 | 4.4 ± 0.2 | 5.6 ± 0.8 | 41.7 ± 1.4 | 1.8 ± 0.3 | 1.04 ± 0.0 |
| ΔcsmA::CSMA | 7.5 ± 2.3 | 53.4 ± 1.7 | 2.8 ± 0.5 | 5.3 ± 0.5 | 5.2 ± 0.2 | 24.1 ± 1.6 | 1.7 ± 0.1 | 2.22 ± 0.2 |
| ΔcsmB::CSMB | 7.8 ± 0.4 | 50.9 ± 0.4 | 3.6 ± 1.0 | 4.4 ± 0.2 | 4.6 ± 0.4 | 26.3 ± 0.1 | 2.5 ± 0.8 | 2.00 ± 0.1 |
| ΔcsmA ΔcsmB::CSMA | 3.0 ± 0.3 | 43.4 ± 0.2 | 1.9 ± 0.1 | 3.2 ± 0.2 | 4.0 ± 0.7 | 42.8 ± 0.8 | 1.6 ± 0.0 | 1.06 ± 0.0 |
| ΔcsmA ΔcsmB::CSMB | 3.6 ± 0.9 | 44.4 ± 2.7 | 2.6 ± 0.6 | 1.4 ± 0.2 | 5.4 ± 1.0 | 39.6 ± 0.8 | 3.0 ± 0.5 | 1.08 ± 0.1 |
a
Data are means ± SD. Man, mannose; Glu, glucose; Gal, galactose; GlcN, glucosamine.