Fig 1.

(A) Induction of the acrAB operon measured by growing E. cloacae Jc194 containing the reporter plasmid pGFP in the presence of 10 mM sodium salicylate (SAL), 10 mM sodium decanoate (DEC), 0.1 mM paraquat (PQ), or 4 μg of tetracycline/ml (TET). The values shown are relative fluorescence units, comparative to the control strain, Jgfp, grown without the compounds. The bars show the average values from triplicate assays. P < 0.05 in all cases. (B) RT-PCR analysis of acrA and acrB gene expression in E. cloacae Jc194 in the presence of 10 mM sodium salicylate (SAL), 10 mM sodium decanoate (DEC), 0.1 mM paraquat (PQ), or 4 μg of tetracycline/ml (TET). The bars show the average values for triplicate assays. The relative expression is calculated as 2^−ΔCT, where ⁻ΔCT is the ratio of the crossing points target value to the crossing point reference value. The target is the strain indicated, whereas the reference is E. cloacae Jc194 in all cases.