Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristicsb | Reference |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| WT | Wild-type P. aeruginosa PAO1 strain H103 | 37 |
| UW-PA3077a | PA3077::ISlacZ/hah-Tcr; insertion at bp 502 (672) in PA3077; derived from UW-WT | 18 |
| UW-PA3078 | PA3078::ISpho/hah-Tcr; insertion at bp 739 (1296) in PA3078; derived from UW-WT | 18 |
| cprR | PA3077::ISlacZ/hah-Tcr, H103 background; Tcr | This study |
| cprS | PA3078::ISpho/hah-Tcr, H103 background; Tcr | This study |
| cprR+ | cprR mutant with Tn7-cprRS+ integrated; Tcr Gmr | This study |
| cprS+ | cprS mutant with Tn7-cprRS+ integrated; Tcr Gmr | This study |
| parR | PA1799::ISlacZ/hah-Tcr, H103 background; Tcr | 10 |
| E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pCR-Blunt II-TOPO | PCR cloning vector; Kanr | Invitrogen |
| pCR-cprRS+ | pCR-Blunt II-TOPO harboring 2.5-kb cprRS amplicon | This study |
| pUC18-mini-Tn7T-Gm | Suicide plasmid; Gmr Ampr | 5 |
| pUC-Tn7-cprRS+ | pUC18-mini-Tn7T-Gm with 2.5-kb cprRS fragment from pCR-cprRS | This study |
| pTNS2 | Transposition helper plasmid; Ampr | 5 |
| pUCPlux-PPA3552 | pUCP23 containing the entire intergenic region between PA3551 and PA3552 fused to luxCDABE | 25 |
a
UW, University of Washington Genome Science Center.
b
Antibiotic resistance phenotypes: Ampr, ampicillin for E. coli and carbenicillin for P. aeruginosa; Gmr, gentamicin; Kanr, kanamycin; Tcr, tetracycline; Strr, streptomycin.