Table 6.
RT-qPCR analysis of the WT as well as the cprR and parR mutants under different conditions
| Strains and conditionsa | Fold change in expression of the indicated gene |
|||
|---|---|---|---|---|
| arnB | pmrB | parR | cprR | |
| WT plus CP28/WT | 335.6 ± 30.4 | 21.6 ± 1.2 | 1.9 ± 0.1 | 0.8 ± 0.1 |
| WT plus CP28/cprR mutant plus CP28 | 58.1 ± 30.8 | 24.4 ± 1.2 | NTb | NT |
| WT plus CP28/parR mutant plus CP28 | 1.1 ± 0.3 | 0.8 ± 0.1 | NT | NT |
| WT plus indolicidin/WT | 150.0 ± 50.1 | 15.19 ± 1.9 | 1.9 ± 0.2 | 1.6 ± 0.1 |
| WT plus indolicidin/cprR mutant plus indolicidin | 4.9 ± 2.6 | 1.34 ± 0.1 | NT | NT |
a
Grown in BM2 medium with or without indolicidin and grown in BM2 and then exposed to 12 μg/ml CP28 for 30 min.
b
NT, not tested.