Fig 1.

Gentamicin-EDTA ALT completely eradicates MSSA biofilm from the implanted TIVAP. ALT was instilled in TIVAP of immunocompetent rats (day 0) and was associated with systemic vancomycin to treat MSSA biofilm colonization (number of rats used for each treatment, 5). ALT was renewed every 24 h for 5 days, and its efficacy was monitored as photon emissions. Results from a representative animal are shown. (A) Control rats with PBS ALT. (B) 70% ethanol ALT. (C) 5 mg/ml gentamicin ALT. (D) 30 mg/ml EDTA alone. (E) Combined gentamicin (5 mg/ml) and EDTA (30 mg/ml) ALT. In panels A to E, representative experiments are shown. (F) Rats were sacrificed after 7 days of treatment, and TIVAP were harvested and monitored for photon emissions. (G) Bacterial cells from TIVAP were harvested and plated on TSB agar for counts of CFU/ml. Results for CFU are means ± standard deviations. Statistical analysis was done using one-way analysis of variance (ANOVA) with Graphpad Prism version 5.0c. A P value of <0.05 was considered significant. **, P ≤ 0.009.