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. 2012 Sep 25;13(10):12232–12241. doi: 10.3390/ijms131012232

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0).

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SDS-PAGE analysis of the recombinant enzyme AFEST. Lane 1: protein marker; Lane 2: the purified recombinant enzyme AFEST. Protein standards employed were: rabbit phosphorylase b (97 kDa), bovine serum albumin (66 kDa), rabbit actin (43 kDa), bovine carbonic anhydrase (31 kDa), and trypsin inhibitor (20 kDa).