Table 6.
Characteristics of the 25 microsatellite loci for wild soybean. Forward (F) and reverse (R) primer sequences, repeat motifs, size range and linkage group are given.
| Primer name | Primer sequence (5′ to 3′) | Repeat motif | Size range | linkage group |
|---|---|---|---|---|
| gmcp1 | F:TCGATTCTATGCCCCTACTT R:AGACTCCCAAGTTTTCAGTCG |
(T)12 | 124–126 | TrnT/trnL |
| gmcp3 | F:GCTTCAGAATTGTCCTATTTA R:ATCAAATAACGCCTCATCTA |
(A)12CG(T)11 | 103–113 | TrnT/trnL |
| gmcp4 | F:TATCACTGTCAAGATTAAGAG R:CTTTTATATGTATGGCGCAAC |
(A)11 | 127–136 | atpB/rbcL |
| RD19 | F:CTAAATATTACAAAATGGAATTCT R:ACCAATTCAAAAAATCGAATA |
(A)14 | 149–151 | rps19 |
| SOYCP | F:CATAGATAGGTACCATCCTTTTT R:CGCCGTATGAAAGCAATAC |
(T)13(G)10 | 90–98 | trnM |
| Satt126 | F:ATAAAACAAATTCGCTGATAT R:GCTTGGTAGCTGTAGGAA |
(ATT)18 | 109–172 | B2 |
| Satt135 | F:TTCCAATACCTCCCAACTAAC R:CACGGATTTTAAATCATTATTACAT |
(ATT)19 | 141–204 | D2 |
| Satt215 | F:GCGCCTTCTTCTGCTAAATCA R:CCCATTCAATTGAGATCCAAAATTAC |
(ATT)11 | 114–221 | J |
| Satt216 | F:TACCCTTAATCACCGGACAA R:AGGGAACTAACACATTTAATCATCA |
(ATT)20 | 137–251 | D1b |
| Satt221 | F:GCGGCAAACCATTATCTTCATT R:GCGATTGTACCACTAAAAACCATAG |
(ATT)23 | 109–224 | D1a |
| Satt231 | F:GGCACGAATCAACATCAAAACTTC R:GCGTGTGCAAAATGTTCATCATCT |
(ATT)32 | 160–328 | E |
| Satt233 | F:AAGCATACTCGTCGTAAC R:GCGGTGCAAAGATATTAGAAA |
(ATT)16 | 169–238 | A2 |
| Satt270 | F:TGTGATGCCCCTTTTCT R:GCGCAGTGCATGGTTTTCTCA |
(ATT)16 | 183–249 | I |
| satt277 | F:GGTGGTGGCGGGTTACTATTACT R:CCACGCTTCAGTTGATTCTTACA |
(ATT)40 | 128–255 | C2 |
| satt288 | F:GCGGGGTGATTTAGTGTTTGACACCT R:GCGCTTATAATTAAGAGCAAAAGAAG |
(ATT)17 | 195–273 | G |
| Satt294 | F:GCGCTCAGTGTGAAAGTTGTTTCTAT R:GCGGGTCAAATGCAAATTATTTTT |
(ATT)23 | 237–303 | C1 |
| Satt373 | F:TCCGCGAGATAAATTCGTAAAAT R:GGCCAGATACCCAAGTTGTACTTGT |
(TAT)21 | 210–279 | L |
| Satt423 | F:TTCGCTTGGGTTCAGTTACTT R:GTTGGGGAATTAAAAAAATG |
(ATT)19 | 225–351 | F |
| Satt463 | F:CTGCAAATTTGATGCACATGTGTCTA R:TTGGATCTCATATTCAAACTTTCAAG |
(ATT)19 | 100–214 | M |
| satt509 | F:GCGCAAGTGGCCAGCTCATCTATT R:GCGCTACCGTGTGGTGGTGTGCTACCT |
(ATT)30 | 119–242 | B1 |
| Satt530 | F:CCAAGCGGGTGAAGAGGTTTTT R:CATGCATATTGACTTCATTATT |
(ATT)12 | 201–279 | N |
| satt555 | F:GCGGTTGGCTTTGATGATGT R:TTACCGCATGTTCTTGGACTA |
(ATT)13 | 234–312 | K |
| Satt568 | F:CGGACACCGGTCTACTAGGAAAGTAA R:GCGGAATAATCCAATTCAATTTA |
(ATT)17 | 212–275 | H |
| satt572 | F:GCGGAGCATGTAAATCCAGCCTATTGA R:GCGGGCTAACTTATGTTACTAAACAAT |
(ATT)14 | 130–241 | A1 |
| satt581 | F:CCAAAGCTGAGCAGCTGATAACT R:CCCTCACTCCTAGATTATTTGTTGT |
(ATT)11 | 130–196 | O |