Figure 2.

Genomic DNA-blot analysis. A, Twenty micrograms of AB15–12-1 genomic DNA was digested with nine restriction enzymes. Lanes from left to right correspond to BamHI, EcoRI, EcoRV, HincII, HindIII, KpnI, PstI, ScaI, and XbaI. B, Ten micrograms of each genomic DNA obtained from leaves of N. sylvestris, N. tomentosiformis, N. tabacum, and suspension-cultured cells of the 5MT^s and 5MT^r tobacco cell line (AB15–12-1) was digested with KpnI. The full-length ASA2 cDNA clone was used as a probe in both A and B. Fragment sizes were determined by a 1-kb ladder (GIBCO-BRL).