Figure 4.

Northern analysis. Twenty micrograms of total RNA per lane was used for northern hybridization. A full-length ASA2 cDNA clone (ASA2) and rRNA (rRNA) were used as probes for hybridization. A, Total RNAs were prepared from 1-week-old N. tabacum suspension-cultured cells (SC) of four 5MT^s (s) and four 5MT^r (r) cell lines, 5MT^s N. tabacum leaves (L) harvested from 3-week-old AB15–12-1 shoot cultures, and 1-week-old 5MT^r N. sylvestris suspension-cultured cells. B, Different plant organs were used to determine tissue specificity. AB15–12-1 leaves, roots, and stems harvested from 3-week-old shoot cultures and dried mature seeds and suspension-cultured 5MT^s cells (TXD) and 5MT^r cells (AB15–12-1) were used to prepare total RNA.