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. 1998 Jun;117(2):533–543. doi: 10.1104/pp.117.2.533

Figure 7.

Figure 7

Nucleotide sequence alignment of ASA2 genomic DNA fragments. The 326-bp ASA2 genomic DNA fragments, corresponding to the ASA2 cDNA sequence from +181 to +392, were amplified from the genomic DNAs of tobacco leaves (ASA2) harvested from AB15–12-1 shoot cultures and three wild-type (5MTs) Nicotiana sp. cultured cell lines, N. tabacum (TXD), N. tomentosiformis (Nto), and N. sylvestris (Ns) using primers 4 (sense) and 10 (antisense), which are shown in bold. These genomic DNA fragments contain a 115-bp intron, which is underlined. Eight nucleotides, indicated by (+) above the sequence, are different in the N. sylvestris genomic fragment compared with those in N. tabacum and N. tomentosiformis. The nucleotide sequences corresponding to Phe-107-Arg-108 are indicated in boldface and underlined with the one-letter amino acid codon abbreviations F and R above the sequence.